A new polymorphic probe on chromosome 22: NB84 (D22S183).

Source/Description: NB84 is a 1.0 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned into pUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 4.6 kb (Al) and 4.0 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization was established by hybridization to a panel of somatic cell hybrids: PgMe-25Nu, containing only human chromosome 22; PgMo-22 and lCB-17ANu, respectively containing both products of the Philadelphia translocation at 22ql 1; A3EW2-3B, containing the t(ll;22) from Ewing's sarcoma (at 22ql2) and 1/22 AM27 containing a t(l;22) at 22ql3 (1,2). The probe was assigned to chromosome 22 between 22pter and 22ql 1. Mendelian Inheritance: Mendelian inheritance has been demonstrated in extended pedigrees of Gilles de la Tourette syndrome families (n = 380). Source/Description: NB129 is a 0.6 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned intopUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 1.6 kb (Al) and 1.3 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization was established by hybridization to a panel of somatic cell hybrids: PgMe-25Nu, containing only human chromosome 22; PgMo-22 and lCB-17ANu, respectively containing both products of the Philadelphia translocation at 22ql 1; A3EW2-3B, containing the t(ll;22) from Ewing's sarcoma (at 22ql2) and 1/22 AM27 containing a t(l;22) at 22ql3 (1, 2). The probe was assigned to the long arm of chromosome 22 between 22qll and 22ql2. Mendelian Inheritance: Mendelian inheritance has been demonstrated in extended pedigrees of Gilles • de la Tourette syndrome families (n = 380).